Surface nutrient regime and bottom hypoxia in Manila Bay during the southwest monsoon

We e amined the surface nutrient regime and hypo ia in Manila Bay, Philippines, during the southwest monsoon along a transect from off Limay, Bataan Peninsula, to Metro Ma nila. The water column showed stratification, with warm, less saline water in the top 10 meters overlying cold, saline bottom water. Hypo ia was present in the bottom waters along the entire transect, and ano ic conditions were observed off Manila. Ammonium concentrations ranged from 6.7 to 40.2 µM, e ceeding those of nitrate and nitrite, both of which were nearly depleted at almost all stations e cept off-Manila, with levels below 0.1 µM. Phosphate varied from 0.1 to 1.9 µM, resulting in a stoichiometrically nitrogen-rich condition at the surface, with N:P ratios ranging from 17.1 to 149.7 and an average of 37.4. This is in contrast to the phosphate-rich con ditions reported during the northeast monsoon period. A plume with high nutrient concentrations and high chlorophyll a was observed off Manila, indicating freshwater inflow from sewage and the Pasig River. Diatoms, including the Skeletonema costatum comple and Chaetoceros spp., were abundant, and these eutrophic conditions likely favored the occurrence of green Noctiluca, while it was not observed during our study.departmental bulletin pape

Papillo-Choledochectomy in the Operative Management of Mucosal Neoplasms of the Periampullary Region

Two patients with mucosal cancer of the periampullary region were treated with papillocholedochectomy, which entails removal of the papilla of Vater and the whole length of the common bile duct. The neoplasm is dissected out through the plane between the duodenal circular and longitudinal muscles, deep to the sphincter of Oddi and the fibromuscular layer of the bile duct. Pathological examination showed that cancer was confined to the mucosal layer without stromal invasion, and that the operation achieved radical cure. For mucosal cancer, papillo-choledochectomy is an alternative to pancreatoduodenectomy, provided that repeated frozen-section studies confirm the completeness of excision

Beyond the frame rate: Measuring high-frequency fluctuations with light intensity modulation

Power spectral density measurements of any sampled signal are typically restricted by both acquisition rate and frequency response limitations of instruments, which can be particularly prohibitive for video-based measurements. We have developed a new method called Intensity Modulation Spectral Analysis (IMSA) that circumvents these limitations, dramatically extending the effective detection bandwidth. We demonstrate this by video-tracking an optically-trapped microsphere while oscillating an LED illumination source. This approach allows us to quantify fluctuations of the microsphere at frequencies over 10 times higher than the Nyquist frequency, mimicking a significantly higher frame rate.Comment: 4 pages, 2 figure

Sphingomyelinase and ceramide inhibit formation of F-actin ring in and bone resorption by rabbit mature osteoclasts

AbstractRecent studies have demonstrated that ceramide plays an important role as a second messenger in many kinds of cells. However, it is not known whether apoptosis of and bone resorption by mature osteoclasts are mediated via sphingomyelinase (SMase) and ceramide. Thus, we examined the possible involvement of SMase and ceramide in the induction of apoptosis in and bone resorption by rabbit mature osteoclasts. SMase and C2-ceramide inhibited strongly F-actin ring formation of and bone resorption by the osteoclasts. However, the osteoclast apoptosis was not induced by C2-ceramide. The ceramide inhibition of the bone resorption was suppressed by dl-threo-dihydrosphingosine, an inhibitor of sphingosine kinase. In addition, we observed that sphingosine-1-phosphate is able to inhibit bone resorption by the osteoclasts. These results suggest an important role of the sphingomyelin pathway in bone resorption by rabbit mature osteoclasts

The discovery channel at the Neutrino Factory: νμ→ντ\nu_\mu\to\nu_\tau pointing to sterile neutrinos

We study the potential of a Neutrino Factory in constraining the parameter space of a scheme with one sterile neutrino separated from three active ones by an O(1) eV^2, mass-squared difference. We present approximated analytic expressions for the oscillation probabilities, showing that the greatest sensitivity to sterile neutrinos at a Neutrino Factory can be achieved using the \nu_\mu ->\nu_\mu and the \nu_\mu ->\nu_\tau oscillations. We have studied two setups: a Neutrino Factory with 50 GeV (20 GeV) stored muons, with two detectors of the Hybrid-MIND type (a magnetized ECC next to a magnetized iron calorimeter), located at L=3000, 7500 km (L=4000, 7500 km) from the source. Four channels have been used: \nu_e -> \nu_\mu,\nu_\tau; \nu_\mu -> \nu_\mu,\nu_\tau. The relevant backgrounds, efficiencies and systematic errors have been taken into account, and we have discussed dependence of the sensitivities on the systematic errors. We have found that the 50 GeV (20 GeV) setup can constrain \sin^2 2 \theta^{(4fam)}_{13} <= 7x10^{-5} (2x10^{-4}); \theta_{34} <= 12 deg (14 deg); and \theta_{24}<= 7.5 deg (8 deg). Our results hold for any value of \Delta m^2_{Sbl} >~ 0.1 eV^2. Eventually we have shown that, if a positive signal is found, the proposed setup is able to measure simultaneously \theta_{34} and \delta_3 with a precision of few degrees and few tens of degrees, respectively, solving the so-called "intrinsic" and "sign degeneracies". Combination of \nu_\mu disappearance and of the \nu_\mu ->\nu_\tau channel, that will be called "the discovery channel", at the two baselines is able to measure at 99% CL a new CP-violating phase \delta_3 for \sin^2 2 \theta_{34} >= 0.06.Comment: [Revised version] 58 pages, 39 figures, uses elsart.cls. Several modifications were mad

In Vivo Imaging of Particle-Induced Inflammation and Osteolysis in the Calvariae of NFκB/Luciferase Transgenic Mice

Wear debris causes biological response which can result in periprosthetic osteolysis after total joint replacement surgery. Nuclear factor-kappa B (NFκB), a representative transcription factor involved in inflammation, is believed to play an important role in this event by regulating the production of proinflammatory mediators and osteoclastogenesis. In this study, we sought to determine whether activation of NFκB in response to stimulation by particles could be visualized by in vivo imaging. We loaded polyethylene (PE) particles onto the calvaria of NFκB/luciferase transgenic mouse, and detected luminescence generated by activation of NFκB. On day 7 after loading, the level of luminescence was maximal. Levels of luminescence were significantly correlated with the levels of luciferase activity, proinflammatory mediator mRNAs, and bone resorption parameters. This system, which enabled us to evaluate particle-induced inflammation and osteolysis without sacrificing mice, constitutes a useful tool for evaluating the efficacy of prophylaxis or treatments for particle-induced osteolysis

Improving the tensile properties of additively manufactured β-containing tial alloys via microstructure control focusing on cellular precipitation reaction

The effect of a two-step heat treatment on the microstructure and high-temperature tensile properties of β-containing Ti-44Al-4Cr (at%) alloys fabricated by electron beam powder bed fusion were examined by focusing on the morphology of α2/γ lamellar grains and β/γ cells precipitated at the lamellar grain boundaries by a cellular precipitation reaction. The alloys subjected to the first heat treatment step at 1573 K in the α + β two-phase region exhibit a non-equilibrium microstructure consisting of the α2/γ lamellar grains with a fine lamellar spacing and a β/γ duplex structure located at the grain boundaries. In the second step of heat treatment, i.e., aging at 1273 K in the β + γ two-phase region, the β/γ cells are discontinuously precipitated from the lamellar grain boundaries due to excess Cr supersaturation in the lamellae. The volume fraction of the cells and lamellar spacing increase with increasing aging time and affect the tensile properties of the alloys. The aged alloys exhibit higher strength and comparable elongation at 1023 K when compared to the as-built alloys. The strength of these alloys is strongly dependent on the volume fraction and lamellar spacing of the α2/γ lamellae. In addition, the morphology of the β/γ cells is also an important factor controlling the fracture mode and ductility of these alloys.Cho K., Odo H., Okamoto K., et al. Improving the tensile properties of additively manufactured β-containing tial alloys via microstructure control focusing on cellular precipitation reaction. Crystals, 11, 7, 809. https://doi.org/10.3390/cryst11070809

Peculiar microstructural evolution and tensile properties of β-containing γ-TiAl alloys fabricated by electron beam melting

The microstructure and tensile properties of β-containing Ti–44Al–4Cr alloy rods additively manufactured by electron beam melting (EBM) process were examined as a function of input energy density determined by the processing parameters. To the best of our knowledge, this is the first report to demonstrate that two types of fine microstructures have been obtained in the β-containing γ-TiAl alloys by varying the energy density during the EBM process. A uniform α2/β/γ mixed structure containing an α2/γ lamellar region and a β/γ dual-phase region is formed at high energy density conditions. On the other hand, a lower energy density leads to the formation of a peculiar layered microstructure perpendicular to the building direction, consisting of a ultrafine α2/γ lamellar grain layer and a α2/β/γ mixed structure layer. The difference in the microstructures originates from the difference in the solidification microstructure and the temperature distribution from the melt pool, which are dependent on the energy density. Furthermore, it was found that the strength of the alloys is closely related to the volume fractions of the β phase and the ultrafine α2/γ lamellar grains which originates from the massive α grains formed by rapid cooling under low energy density conditions. The alloys with high amounts of these peculiar microstructures exhibit high strength comparable to and higher than the conventional β-containing γ-TiAl at room temperature and 1023 K, respectively.Cho K., Kawabata H., Hayashi T., et al. Peculiar microstructural evolution and tensile properties of β-containing γ-TiAl alloys fabricated by electron beam melting. Additive Manufacturing, 46, 102091. https://doi.org/10.1016/j.addma.2021.102091